Journal: eLife

Article Title: Cell lineage and cell cycling analyses of the 4d micromere using live imaging in the marine annelid Platynereis dumerilii

doi: 10.7554/eLife.30463

Figure Lengend Snippet: ( A ) A Zeiss inverted scope with a gliding stage, coupled with the Eppendorf Femtojet microinjector and Eppendorf Transferman micromanipulation system were used for injections. Femtotip ready-to-use injection needles were back-filled with injection solution ( A’ ). An agarose platform ( A’’ ) with a groove large enough to contain embryos was prepared using a plastic custom-made mold. Under a dissecting scope, small incisions were made to the right side of the groove ( A’’’ ), and these incisions were used to remove embryos from the needle by sliding the needle through them. The agarose platform was placed into a small petri dish lid and covered with filtered sea water before the embryos were transferred into the dish. In B and C, the steps of general experimental outline for live imaging samples at different stages are listed.

Article Snippet: Microinjections were carried out using an inverted Zeiss Axiovert 135 scope, Eppendorf Femtojet 4i, and Eppendorf Transferman Micromanipulator ( ).

Techniques: Micromanipulation, Injection, Imaging